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PCR-amplified ORFs were inserted into a variant of vector 438-C of the 438 series of MacroLab vectors (modified pFastBac, Addgene plasmid # 48296) 65
Common adverse effects include gastrointestinal symptoms such as nausea, vomiting, diarrhea, constipation, and abdominal pain
The present study observed reduced expression of 25-hydroxylase enzymes and 1--hydroxylase, vitamin D receptor, and increased expression of the 24-hydroxylase enzyme in the livers of HFD-fed mice
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